TY - JOUR
T1 - Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis
AU - Montoya, Y.
AU - Leon, C.
AU - Talledo, M.
AU - Nolasco, O.
AU - Padilla, C.
AU - Muñoz-Najar, U.
AU - Barker, D. C.
N1 - Funding Information:
D. C. Barker is financially supported by the Medical Research Council, UK. Y. Montoya received support from a Research Training Grant provided by the UNDPWorld Bank WHO Special Programme for Research and Training in Tropical Diseases. This work also received fmancial support from the STD-3 Programme of the European Community, contract no. TS3*CT92-0123.
PY - 1997
Y1 - 1997
N2 - The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4:) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.
AB - The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4:) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.
KW - Diagnosis
KW - Leishmania peruviana
KW - Leishmaniasis
KW - Recombinant proteins
UR - http://www.scopus.com/inward/record.url?scp=0031473994&partnerID=8YFLogxK
U2 - 10.1016/S0035-9203(97)90520-4
DO - 10.1016/S0035-9203(97)90520-4
M3 - Article
C2 - 9580116
AN - SCOPUS:0031473994
SN - 0035-9203
VL - 91
SP - 674
EP - 676
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 6
ER -
