Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts; Oscar Nolasco; Pim De Rijk; Elie Nduwamahoro; Armand Van Deun; Andy Ramsay; Jorge Arevalo; Françoise Portaels

doi:10.1128/JCM.01489-06

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts, Oscar Nolasco, Pim De Rijk, Elie Nduwamahoro, Armand Van Deun, Andy Ramsay, Jorge Arevalo, Françoise Portaels

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

41 Citas (Scopus)

Resumen

New rpoB gene primers for detecting Rif^r in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif^r isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.

Idioma original	Inglés
Páginas (desde-hasta)	252-254
Número de páginas	3
Publicación	Journal of Clinical Microbiology
Volumen	45
N.º	1
DOI	https://doi.org/10.1128/JCM.01489-06
Estado	Publicada - ene. 2007
Publicado de forma externa	Sí

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Acceder al documento

10.1128/JCM.01489-06

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{3f6e970b802b45858140f09a81803e03,

title = "Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis",

abstract = "New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.",

author = "Leen Rigouts and Oscar Nolasco and {De Rijk}, Pim and Elie Nduwamahoro and {Van Deun}, Armand and Andy Ramsay and Jorge Arevalo and Fran{\c c}oise Portaels",

year = "2007",

month = jan,

doi = "10.1128/JCM.01489-06",

language = "English",

volume = "45",

pages = "252--254",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "1",

}

TY - JOUR

T1 - Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

AU - Rigouts, Leen

AU - Nolasco, Oscar

AU - De Rijk, Pim

AU - Nduwamahoro, Elie

AU - Van Deun, Armand

AU - Ramsay, Andy

AU - Arevalo, Jorge

AU - Portaels, Françoise

PY - 2007/1

Y1 - 2007/1

N2 - New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.

AB - New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanel-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster 1,2% at codon 176, and 1% at codon 497.

UR - http://www.scopus.com/inward/record.url?scp=33846248286&partnerID=8YFLogxK

U2 - 10.1128/JCM.01489-06

DO - 10.1128/JCM.01489-06

M3 - Article

C2 - 17093024

AN - SCOPUS:33846248286

SN - 0095-1137

VL - 45

SP - 252

EP - 254

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 1

ER -

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Resumen

ODS de las Naciones Unidas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto